A novel versatile flow-donor chamber as biorelevant ex-vivo test assessing oral mucoadhesive formulations
نویسندگان
چکیده
Oral transmucosal drug delivery is a non-invasive administration route for rapid therapeutic onset and greater bioavailability avoiding the first-pass metabolism. Mucoadhesive formulations are advantageous as they may retain at site. Proper equipment to assess mucoadhesive properties corresponding absorption fundamental development of novel systems. Here we developed new flow-through donor chamber well-established diffusion cells, tested effects on formulation retention in situ adding polymers or mesoporous silica particles reference formulation. Mesoporous particular interest be used encapsulate molecules. Compared other ex-vivo methods described literature assessing performance delivery, this provides several advantages: i) it reflects physiological conditions better realistic saliva flow can provided over site, ii) versatile since mounted any kind vertical cell allowing simultaneous detection site permeation through tissue, iii) enables optical quantification residence time aided by image processing. This set-up proved sensitive differentiate from one where 20 %(w/w) Carbomer was added (to further improve properties), with respect both times. We also found that particles, investigated only mixed together formulation, gave very similar what observed Carbomer. However, after some (>30 min) became obvious tablet excipients promote particle mucosa. work simple biorelevant test evaluation oral paves way studies valuable enhancing mucoadhesion.
منابع مشابه
Assessing Leukocyte-endothelial Interactions Under Flow Conditions in an Ex Vivo Autoperfused Microflow Chamber Assay
Leukocyte-endothelial interactions are early and critical events in acute and chronic inflammation and can, when dysregulated, mediate tissue injury leading to permanent pathological damage. Existing conventional assays allow the analysis of leukocyte adhesion molecules only after the extraction of leukocytes from the blood. This requires the blood to undergo several steps before peripheral blo...
متن کاملBiorelevant Dissolution Methods and Their Applications in In Vitro- In Vivo Correlations for Oral Formulations
Dissolution tests that can predict the in vivo performance of drug products are usually called biorelevant dissolution tests. Biorelevant dissolution testing can be used to guide formulation development, to identify food effects on the dissolution and bioavailability of orally administered drugs, and to identify solubility limitations and stability issues. To develop a biorelevant dissolution t...
متن کاملIn vivo evaluation of mucoadhesive properties of nanoliposomal formulations upon coating with trimethylchitosan polymer
Objective(s): Drug delivery via mucosal routes has been confirmed to be effective in inducing strong immune responses. Liposomes could enhance immune responses and mucoadhesive potentials, make them useful mucosal drug delivery systems. Coating of liposomes by mucoadhesive polymers succeeded in enhancing immune responses. Our studies aim at preparation and characterization of trimethylchitosan-...
متن کاملDissolution of Pentoxifylline from Extended Release Formulations. Researches Concerning Development of a Biorelevant Test
This paper presents results of a pharmacokinetics study concerning pentoxifylline and its main metabolites after administration of extended release formulation of Trental 400 mg and correlation of this pharmacokinetics with in vitro dissolution test results of parent drug. In order to establish most relevant in vitro test, dissolution was performed in different experimental conditions (stirring...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: European Journal of Pharmaceutical Sciences
سال: 2021
ISSN: ['0928-0987', '1879-0720']
DOI: https://doi.org/10.1016/j.ejps.2021.105983